Coding

Part:BBa_K3190102:Design

Designed by: Ojas Tulsyan   Group: iGEM19_UCopenhagen   (2019-10-13)


G protein-coupled estrogen receptor (GPER/GPR30) CDS tagged with myc


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 750


Design Notes

The myc tag was added to the C-terminus of the receptor in order to confirm the protein expression.


Source

Homo sapiens

References